WebLiao and Seebeck recently reported the enzymatic synthesis of SAM from SAH and methyl iodide, using a halide methyltransferase (HMT) from Chloracidobacterium thermophilum … WebJan 1, 2024 · Fluorescently labeled compounds are useful tools for high-throughput screenings of large compound libraries. Here we utilized the azaindole-based scaffold of FDA-approved BRAF inhibitor vemurafenib 1, which displays off-target activity on MKK4, as a starting point in our fluorescent compound design. Chemical variation of the scaffold and ...
Enzymatic stereospecific preparation of fluorescent S-adenosyl-L ...
WebOct 27, 2024 · Liao and Seebeck recently reported the enzymatic synthesis of SAM from SAH and methyl iodide, using a halide methyltransferase (HMT) from … WebHere, we describe the synthesis of stable and fluorescent analogs of SAM in which the fluorophore is introduced through a functionalizable linker to the ribose. A Cy5-labeled SAM analog was shown to bind several SAM-I riboswitches via in-line probing and fluorescence polarization assays, including one from Staphylococcus aureus that controls the … chroot . sh illegal instruction
Construction of Fluorescent Analogs to Follow the Uptake …
WebGangliosides, glycosphingolipids containing one or more sialic acids in the glycan chain, are involved in various important biological processes in cell plasma membranes (PMs). However, the behaviors and functions of gangliosides are poorly understood, primarily because of the lack of fluorescent an … WebMar 17, 2024 · In this work, the preparation of new S-adenosyl-l-methionine (SAM) analogues for sequence specific DNA labeling is evaluated. These non-natural analogues, comprising cysteine rather than the natural homolog, were obtained in near quantitative conversions from readily available starting materials without relying on using an excess … WebAug 16, 2024 · The transformation of uroporphyrinogen III into a corrin involves, among other events, the addition of 8 S-adenosyl-L-methionine (SAM)-derived methyl groups, the … dermatome and cutaneous nerve