WebNov 10, 2024 · A viral replicon system suited to the stable replication of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), without cytopathic effects, has been reported recently in a study... WebWe present a temperature-regulated, alphavirus replicon-based DNA expression system. The system is regulated by a viral temperature-sensitive RNA-dependent RNA replicase, creating a temperature-dependent RNA amplification loop. Because of this positive feedback, the system exhibits both low background and high inducibility.
Engineering a Reliable and Convenient SARS-CoV-2 Replicon
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Cell-based viral replication system for screening of SARS …
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